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Imaging Mass Spectrometry Protocols for Mass Microscopy

:	Soeren-Oliver Deininger, Masaru Furuta, Naoko Goto-Inoue, John S. Hammond, Takahiro Hayasaka, Daisuk
:	Mitsutoshi Setou
:	Imaging Mass Spectrometry Protocols for Mass Microscopy
:	Springer-Verlag
:	9784431094258
:	1
:	CHF 89.50
:

:	Allgemeines, Lexika
:	English

:	272
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

Addressing the widespread need for a practical guide to imaging mass spectrometry (IMS), this book presents the protocols of IMS technology. As that technology expands, research groups around the world continue its development. Pharmaceutical companies are using IMS for drug analyses to study pharmacokinetics and medical properties of drugs. Drug research and disease-related biomarker screening are experiencing greater use of this technology, with a concurrent increase in the number of researchers in academia and industry interested in wider applications of IMS. Intended for beginners or those with limited experience with IMS technology, this book provides practical details and instructions needed for immediate know-how, including the preparation of animal tissue samples, the application of a matrix, instrumental operations, and data analysis, among others. By describing the foundations of IMS, this volume contributes to the ongoing development of the field and to progress in human health.

	Preface	5
	Contents	6
	Contributors	8
	Part I: Innovation of Imaging Mass Spectrometry	10
	Chapter 1	11
	IMS as an Historical Innovation	11
	References	15
	Part II: Planning the Sample Preparation	16
	Chapter 2	17
	Guide to Planning the Sample Preparation Step	17
	2.1 Introduction	17
	2.2 Characteristics of MALDI in Direct Tissue Mass Spectrometry	18
	2.3 IMS for Proteins and Peptides	20
	2.3.1 On-Tissue Digestion Method	24
	2.3.2 Application to Protein Studies	24
	2.4 IMS for Small Organic Compounds	25
	2.4.1 IMS for Endogenous Metabolites	29
	2.4.2 Application to Lipid Studies	30
	2.4.3 Imaging of Administered Drugs	30
	2.5 Conclusion	34
	References	35
	Part III: Sample Preparation	37
	Chapter 3	38
	Animal Care and Tissue Sample Extraction for IMS	38
	3.1 Methods of Mouse Euthanasia	38
	3.1.1 Euthanasia by Anesthetics	38
	3.1.2 Cervical Dislocation	39
	3.1.3 Decapitation	39
	3.2 Anatomy of the Mouse	39
	3.3 Freezing of the Mouse Brain	40
	3.3.1 Materials	40
	3.3.2 Procedure	41
	3.4 Samples Other Than Mouse Brain	42
	3.5 Evaluation of the Time Elapsed After Death in Animals	43
	Chapter 4	45
	Preparing Biological Tissue Sections for Imaging Mass Spectrometry	45
	4.1 Introduction	45
	4.2 Embedding	47
	4.3 Excision of Thin Slices	48
	4.4 Section Support Materials	49
	4.5 Postmortem Degradation	50
	4.6 Rinsing of Tissue Sections with Organic Solvents	53
	4.7 Staining of Tissue Sections Before IMS Measurement	55
	4.8 A Standard Procedure for Preparing Frozen Sections	56
	4.8.1 Materials	56
	4.8.2 Methods	56
	4.8.3 Notes	56
	4.9 Conclusion	57
	References	57
	Chapter 5	59
	Matrix Choice	59
	5.1 Principle of Molecular Ionization	59
	5.2 Choice of Matrices	60
	5.2.1 Ionic Matrices	61
	5.2.2 Challenges for Imaging of Primary Metabolites in m / z	61
	62	61
	5.2.3 Nanoparticle-Based IMS	63
	5.3 Composition of Matrix Solvent	66
	References	72
	Chapter 6	74
	Methods of Matrix Application	74
	6.1 Introduction	74
	6.2 Manual Spray-Coating	75
	6.2.1 Protocol of Spraying Operation with an Artistic Air-Brush	76
	6.2.2 Preparation of Matrix Solution	77
	6.2.3 Matrix Application with an Artistic Air-Brush	77
	Note	77
	6.2.4 Other Notes	79
	6.3 Automatic Dispensing of Small Droplets by the ChiP Instrument	79
	6.3.1 On-Tissue Digestion and Matrix Application with the ChIP-1000 Instrument	80
	6.3.1.1 Preparation of Trypsin Stock Solution	81
	6.3.2.1 Matrix Application Using the ChIP-1000	81
	6.4 Spray-Droplet Method	82
	6.4.1 Protocol	83
	6.4.2 Notes Regarding the Spray-Droplet Method	86
	6.5 Matrix Sublimation Method	86
	References	88
	Chapter 7	89
	Matrix Applicadtion with ImagePrep	89
	7.1 Introduction	89
	7.2 The Simple GUI	91
	7.3 The Operation	91
	7.4 The Optical Sensor	91
	7.5 The Method Development GUI	92
	Part IV: Instrumental Operation for IMS	94
	Chapter 8	95
	Method of Operating AXIMA-QIT as Imaging Instrument	95
	8.1 Outline of AXIMA-QIT-TOF-MS	95
	8.2 IMS by AXIMA-QIT-TOF-MS	96
	8.2.1 Acquisition of Position Information by ChIP-1000	96
	8.2.2 Settings in AXIMA-QIT-TOF-MS	100
	8.2.3 Achieving Settings in AXIMA-QIT-TOF-MS	102
	8.2.4 Calibration	102
	8.2.5 Setting of Measurement Area	103
	8.2.6 IMS Measurement	104
	8.3 Tandem MS by AXIMA-QIT-TOF-MS	105
	8.3.1 Selection of Peak	106
	8.3.2 MS 2 Measurement	107
	8.3.3 Identification of Peptide	108
	Part V: Analysis of Measurement Data	110