| Series Editor | 5 |
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| Preface | 6 |
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| Contents | 8 |
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| Contributors | 16 |
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| Part 1 Fluorescence Spectroscopy: Basics and Advanced Approaches | 19 |
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| 1 Basics of Fluorescence Spectroscopy in Biosciences | 20 |
| 2 Pulse and Phase Fluorometries: An Objective Comparison | 47 |
| 3 Non- Exponential Fluorescence of Electronically Coupled Donors Contains Distance Information | 66 |
| 4 Fluorescence Nanotomography: Recent Progress, Constraints and Opportunities | 73 |
| 5 Solvent Relaxation as a Tool for Probing Micro- Polarity and - Fluidity | 88 |
| 6 Total Internal Reflection Fluorescence Microscopy: Applications in Biophysics | 96 |
| 7 Single Molecule Spectroscopy: Basics and Applications | 121 |
| Part 2 Application of Fluorescence Spectroscopy to Biological Membranes | 148 |
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| 8 Raft Microdomains in Model Membranes as Revealed by Fluorescence Quenching | 149 |
| 9 The Lateral Structure of Lipid Membranes as Seen by Fluorescence Microscopy | 166 |
| Part 3 Application of Fluorescence Spectroscopy to Protein Studies | 176 |
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| 10 Protein Dynamics and Protein Folding Dynamics Revealed by Time- Resolved Fluorescence | 177 |
| 11 Time- Resolved Fluorescence and Two- Photon FCS Investigation of the Interaction of HIV- 1 Nucleocapsid Protein with Hairpin Loop Oligonucleotides | 194 |
| Part 4 Application of Fluorescence Spectroscopy to DNA and Drug Delivery | 212 |
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| 12 Fluorescence Techniques in Non- Viral Gene Therapy | 213 |
| 13 Fluorescence Applications in Targeted Drug Delivery | 241 |
| Part 5 Fluorescence Spectroscopy in cells: FCS and Quantum Dots | 255 |
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| 14 Fluorescence Correlation Spectroscopy in Cell Biology | 256 |
| 15 Fluorescent Quantum Dots: Properties and Applications | 274 |
| 16 Heat Stress of Cancer Cells: Fluorescence Imaging of Structural Changes with Quantum Dots 605 and Alexa 488 | 286 |
| Sachverzeichnis | 299 |
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