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Agrobacterium: From Biology to Biotechnology From Biology to Biotechnology

:	Tzvi Tzfira, Vitaly Citovsky
:	Tzvi Tzfira, Vitaly Citovsky
:	Agrobacterium: From Biology to Biotechnology From Biology to Biotechnology
:	Springer-Verlag
:	9780387722900
:	1
:	CHF 165.10
:

:	Botanik
:	English

:	750
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

Agrobacterium is a plant pathogen which causes the 'crown-gall' disease, a neoplastic growth that results from the transfer of a well-defined DNA segment ('transferred DNA', or 'T-DNA') from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications.

The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.

Dr. Tzvi Tzfira is an Assistant Professor in the Department of Molecular, Cellular, and Developmental Biology at the University of Michigan.

Dr. Vitaly Citovsky is a Professor in the Department of Biochemistry and Cell Biology at Stony Brook University.

	Contributing Authors	6
	Table of Contents	11
	Preface	27
	Color Figures	29
	Acknowledgments	30
	AGROBACTERIUM: A DISEASE-CAUSING BACTERIUM	31
	1 INTRODUCTION	32
	2 AGROBACTERIUM HOST RANGE	34
	3 DIVERSITY OF NATURAL ISOLATES	35
	4 SOURCES OF INFECTION AND CONTROL OF CROWN GALL DISEASE	42
	5 ACKNOWLEDGEMENTS	56
	6 REFERENCES	56
	A BRIEF HISTORY OF RESEARCH ON AGROBACTERIUM TUMEFACIENS: 1900-1980s	77
	1 INTRODUCTION	77
	2 AGROBACTERIUM— THE PATHOGEN	79
	3 A. TUMEFACIENS AS THE VECTOR OF CHOICE FOR PLANT GENETIC ENGINEERING	89
	4 CONCLUSIONS	94
	5 ACKNOWLEDGEMENTS	94
	6 REFERENCES	95
	AGROBACTERIUM AND PLANT BIOTECHNOLOGY	103
	1 INTRODUCTION	104
	2 THE DEVELOPMENT OF AGROBACTERIUM-MEDIATED TRANSFORMATION	105
	3 APPLICATIONS OF AGROBACTERIUM-MEDIATED TRANSFORMATION	121
	4 GENE FLOW AND MOLECULAR APPROACHES TO TRANSGENE CONTAINMENT/ MONITORING	143
	5 GLOBAL STATUS OF AGRICULTURAL BIOTECHNOLOGY AND TECHNOLOGY TRANSFER	146
	6 ACKNOWLEDGEMENTS	152
	7 REFERENCES	152
	THE AGROBACTERIUM TUMEFACIENS C58 GENOME	178
	1 INTRODUCTION	179
	2 GENERAL FEATURES OF THE GENOME	179
	3 THE LINEAR CHROMOSOME	181
	4 PHYLOGENY AND WHOLE-GENOME COMPARISON	184
	5 DNA REPLICATION AND THE CELL CYCLE	185
	6 GENUS-SPECIFIC GENES	186
	7 PLANT TRANSFORMATION AND TUMORIGENESIS	187
	8 TRANSPORT	188
	9 REGULATION	189
	10 RESPONSE TO PLANT DEFENSES	191
	11 GENERAL METABOLISM	192
	12 CONCLUSIONS	195
	13 ACKNOWLEDGEMENTS	198
	14 REFERENCES	198
	AGROBACTERIUM— TAXONOMY OF PLANT- PATHOGENIC RHIZOBIUM SPECIES	211
	1 INTRODUCTION	212
	2 HISTORICAL PERSPECTIVE— ORIGINS	213
	3 AGROBACTERIUM—RHIZOBIUM RELATIONSHIPS	223
	4 GENOTYPIC RELATIONSHIPS	224
	5 PLASMID TRANSFER AND GENUS RECLASSIFICATION	227
	6 DIVERSITY WITHIN RHIZOBIUM	228
	7 REVISION OF AGROBACTERIUM NOMENCLATURE	232
	8 RELATIONSHIP OF RHIZOBIUM TO OTHER MEMBERS OF THE RHIZOBIACEAE	235
	9 OTHER ‘ AGROBACTERIUM’ SPECIES	236
	10 SUMMARY	237
	11 ACKNOWLEDGEMENTS	237
	12 REFERENCES	238
	THE INITIAL STEPS IN AGROBACTERIUM TUMEFACIENS PATHOGENESIS: CHEMICAL BIOLOGY OF HOST RECOGNITION	249
	1 INTRODUCTION	250
	2 SIGNAL DIVERSITY	251
	3 SIGNAL RECOGNITION, INTEGRATION AND TRANSMISSION	254
	4 SUMMARY	264
	5 ACKNOWLEDGEMENTS	264
	6 REFERENCES	264
	AGROBACTERIUM-HOST ATTACHMENT AND BIOFILM FORMATION	270
	1 INTRODUCTION	271
	2 PRESUMPTIVE ADHERENCE FACTORS	274
	3 PLANT RECEPTORS RECOGNIZED DURING A. TUMEFACIENS INFECTION	285
	4 BIOFILM FORMATION BY A. TUMEFACIENS	286
	5 A MODEL FOR ADHERENCE AND BIOFILM FORMATION	293
	6 A WIDE RANGE OF SURFACE INTERACTIONS	294
	7 CONCLUSIONS	295
	8 ACKNOWLEDGEMENTS	296
	9 REFERENCES	296
	PRODUCTION OF A MOBILE T-DNA BY AGROBACTERIUM TUMEFACIENS	305
	1 INTRODUCTION	306
	2 A. TUMEFACIENS—NATURE’S GENETIC ENGINEER	306
	3 INTERKINGDOM GENE TRANSFER	307
	4 VirD2 INTERACTS WITH HOST PROTEINS	320
	5 T-DNA INTEGRATION	323
	6 PLANT GENETIC ENGINEERING	325
	7 ACKNOWLEDGEMENTS	327
	8 REFERENCES	327
	TRANSLOCATION OF ONCOGENIC T-DNA AND EFFECTOR PROTEINS TO PLANT CELLS	340
	1 INTRODUCTION	341
	2 A HISTORICAL OVERVIEW	341
	3 A. TUMEFACIENS VIRB/D4 SECRETION SUBSTRATES	345
	4 THE VIRB/D4 MACHINE	351
	5 VIRB/D4 MACHINE ASSEMBLY AND SPATIAL POSITIONING	358
	6 VIRB/D4 CHANNEL/PILUS ARCHITECTURE	364
	7 T-DNA TRANSLOCATION ACROSS THE CELL ENVELOPE	366
	8 THE AGROBACTERIUM – PLANT CELL INTERFACE	372
	9 SUMMARY AND PERSPECTIVES	375
	10 ACKNOWLEDGEMENTS	377
	11 REFERENCES	377
	INTRACELLULAR TRANSPORT OF AGROBACTERIUM T- DNA	390
	1 INTRODUCTION	390
	2 STRUCTURE AND FUNCTION OF THE T-COMPLEX	392
	3 CYTOPLASMIC TRANSPORT	397
	4 NUCLEAR IMPORT	399
	5 INTRANUCLEAR MOVEMENT OF THE T-COMPLEX	406
	6 FROM THE CYTOPLASM TO THE CHROMATIN: A MODEL FOR T- COMPLEX IMPORT	407
	7 FUTURE PROSPECTS	409
	8 ACKNOWLEDGEMENTS	409
	9 REFERENCES	410
	MECHANISMS OF T-DNA INTEGRATION	420
	1 INTRODUCTION	421
	2 THE T-DNA MOLECULE	422
	3 PROTEINS INVOLVED IN T-DNA INTEGRATION	423
	4 GENOMIC ASPECTS OF T-DNA INTEGRATION/ TARGET- SITE SELECTION	433
	5 MODELS FOR T-DNA INTEGRATION	445
	6 FUTURE DIRECTIONS	453
	7 ACKNOWLEDGEMENTS	454
	8 REFERENCES	454
	AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION: PATTERNS OF T- DNA INTEGRATION INTO THE HOST GENOME	466
	1 INTRODUCTION	467
	2 T-DNA INTEGRATION MECHANISM: SUCCESSIVE STEPS LEADING TO STABLE INTEGRATION OF THE T- DNA INTO THE PLANT HOST GENOME	468
	3 PATTERNS OF T-DNA INTEGRATION INTO THE HOST GENOME	483
	4 ACKNOWLEDGMENTS	494
	5 REFERENCES	494
	FUNCTION OF HOST PROTEINS IN THE AGROBACTERIUM- MEDIATED PLANT TRANSFORMATION PROCESS	507
	1 INTRODUCTION	508
	2 A GENETIC BASIS EXISTS FOR HOST SUSCEPTIBILITY TO AGROBACTERIUM-MEDIATED TRANSFORMATION	509
	3 THE PLANT RESPONSE TO AGROBACTERIUM: STEPS IN THE TRANSFORMATION PROCESS, AND PLANT GENES/ PROTEINS INVOLVED IN EACH OF THESE STEPS	512