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Flow Cytometry in Hematopathology A Visual Approach to Data Analysis and Interpretation

:	Doyen T. Nguyen, Lawrence W. Diamond, Raul C. Braylan
:	Flow Cytometry in Hematopathology A Visual Approach to Data Analysis and Interpretation
:	Humana Press
:	9781597451628
:	2
:	CHF 151.90
:

:	Klinische Fächer
:	English

:	344
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

The second edition of this volume reflects the recent advances in the FCM analysis of hematopoietic disorders. The chapters have been revised to incorporate new text and figures. The volume is aimed at hematopathologists, hematologists, pathologists, and laboratory technicians.

	Contents	6
	Preface to the Second Edition	10
	Preface to the First Edition	11
	Acknowledgments	13
	List of Abbreviations	14
	List of Case Studies	16
	1 Approach to flow cytometry: General considerations	19
	1.1 Reasons for the necessity of proper data analysis	20
	1.2 General aspects of FCM data analysis and interpretation	22
	1.3 Other applications of FCM in hematopathology	25
	1.4 Maturation and differentiation of hematopoietic elements: An overview based on the immunologic markers currently in use in the FCM laboratory	27
	2 FCM immunophenotyping and DNA analysis: Practical aspects that can affect data analysis and interpretation	31
	2.1 Sample selection	31
	2.1.1 Liquid specimens	32
	2.1.2 Solid tissue specimens	33
	2.2 Preparing nucleated cell suspensions	34
	2.3 Cell yield and viability	34
	2.4 Sample staining	34
	2.4.1 Surface antigens	35
	2.4.2 Intracellular antigens	35
	2.4.3 DNA content	36
	2.5 Data acquisition	37
	2.5.1 Calibration	37
	2.5.2 Color compensation	37
	2.5.3 List mode data collection	38
	2.5.4 Exclusion of nonviable cells	39
	2.6 Antibody panel design	40
	2.6.1 Antibody selection	41
	2.6.2 Fluorochrome conjugation	44
	2.7 Comprehensive antibody panels	49
	2.7.1 Disease-oriented antibody panels	49
	2.7.2 Antibody panels oriented by specimen type	50
	2.8 Tailored panels and add-on testing	52
	2.9 FCM immunophenotyping data representation	55
	2.9.1 Analysis panels	55
	2.9.2 Color display	56
	2.10 Approach to DNA data analysis	59
	2.10.1 DNA ploidy	60
	2.10.2 S-phase	60
	3 FCM data analysis on nearly homogeneous samples	67
	3.1 FCM parameters	68
	3.1.1 Forward scatter	68
	3.1.2 Side scatter	71
	3.1.3 Fluorescence	72
	3.2 Fluorescence dynamic range	89
	3.3 Strategy to the visual review of FCM immunophenotyping data	89
	3.4 Common SSC/CD45 patterns	91
	3.4.1 Assessment of the blast population	91
	3.4.2 Immature neoplastic cells with downregulated CD45	99
	3.4.3 SSC/CD45 in mature lymphoid disorders	101
	3.5 Other dot plot patterns useful in acute leukemia diagnosis	104
	3.5.1 Useful antigenic features in AML	104
	3.5.2 Precursor B-ALL versus bone marrow B-cell progenitors	116
	3.5.3 Useful antigenic features in precursor T-lymphoma/leukemia	117
	3.6 Evaluation of mature lymphoid malignancies	124
	3.6.1 Assessment of surface light chain expression	124
	3.6.2 Assessment of pan B-cell antigens	126
	3.6.3 Useful antigenic features in mature B-cell malignancies	130
	3.6.4 Identification of abnormal mature T-cells	149
	3.6.5 Useful antigenic features in mature T-cell malignancies	168
	3.7 Assessing the biological behavior of mature lymphoid neoplasms	184
	3.8 Dot plot patterns in histiocytic proliferations and nonhematopoietic malignancies	190
	Plates	192
	4 FCM data analysis on heterogeneous specimens	209
	4.1 Identifying normal FCM samples	209
	4.2 Abnormal heterogeneous samples with a detectable immature neoplastic population	254
	4.2.1 Blasts of lymphoid lineage	255
	4.2.2 Blasts of myeloid lineage	256
	4.3 Minimal residual disease	267
	4.4 Abnormal heterogeneous samples with detectable mature neoplastic populations	267
	4.4.1 Abnormal mature B-cells	272
	4.4.2 Abnormal mature T-cells and NK cells	279
	4.4.3 Abnormal plasma cells present	289
	4.5 Abnormal blood or bone marrow samples with no detectable neoplastic cells	294
	4.5.1 Altered cellular composition and abnormal SSC	296
	4.5.2 Abnormal antigenic maturation in myeloid or erythroid precursors	307
	4.6 Coexisting malignancies	319
	5 FCM interpretation and reporting	322
	5.1 Immature hematopoietic malignancies	322
	5.1.1 ALL/lymphoblastic lymphoma	323
	5.1.2 Myeloid malignancies	325
	5.1.3 Acute leukemias with a multilineage antigenic pro.le	333
	5.2 Mature lymphoid malignancies	333
	5.2.1 B-cell LPD/NHL	335
	5.2.2 Plasma cell dyscrasias	351
	5.2.3 T-cell LPD/NHL	353
	5.3 FCM reporting	366
	Suggested reading	367
	Using the case studies	373
	Limited warranty and disclaimer	373
	Appendix: Using the case studies CD-ROM	373
	Index	374