: Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters
: Regulation of Gene Expression
: Humana Press
: 9781597452281
: 1
: CHF 85,30
:
: Biochemie, Biophysik
: English
: 334
: Wasserzeichen/DRM
: PC/MAC/eReader/Tablet
: PDF
The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select 'Courses. ' The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.
Preface5
Contents7
COMPANION CD9
Part I GENE EXPRESSION CONTROL AT THE MRNA LEVEL10
Messenger RNA Accumulation20
1. Concepts20
2. Basic Methods and Approaches23
3. Summary38
Transcript Profiling40
1. Concepts40
2. Methods and Approaches42
3. Summary57
Transcriptional Regulation of Gene Expression58
1. Concepts58
2. Methods and Approaches69
3. Summary107
Posttranscriptional Processing of Messenger RNA114
1. Concepts114
2. Methods and Approaches125
3. Summary145
Study Questions150
References152
Part II REGULATION OF PROTEIN LEVELS AND TRANSCRIPTION FACTOR FUNCTION156
Overview158
Protein Synthesis and Turnover164
1. Concepts164
2. Methods and Approaches166
3. Summary168
Proteomics170
1. Concepts170
2. Methods and Approaches171
3. Summary181
Determination of Protein–Protein Interactions and the Motifs That Mediate Them182
1. Concepts182
2. Methods and Approaches182
3. Summary223
Posttranslational Modifications224
1. Concepts224
2. Methods and Approaches229
3. Summary246
Dissection of Signaling Pathways248
1. Concepts248
2. Methods and Approaches249
3. Summary266
Study Questions268
References270
Part III USE OF TRANSGENIC AND KNOCKOUT MICE TO STUDY GENE REGULATION274
OVERVIEW276
Production of Transgenic Animals280
1. Standard Production280
2. Applications of Transgenic Models287
KNOCKOUT ANIMALS304
1. Standard Production304
2. Applications of Null Mouse Models309
Isolation of Cells and Cell Lines From Transgenic and Knockout Animals328
1. Standard Production328
Study Questions330
References332
Index338