| Preface | 5 |
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| Contents | 7 |
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| COMPANION CD | 9 |
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| Part I GENE EXPRESSION CONTROL AT THE MRNA LEVEL | 10 |
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| Messenger RNA Accumulation | 20 |
| 1. Concepts | 20 |
| 2. Basic Methods and Approaches | 23 |
| 3. Summary | 38 |
| Transcript Profiling | 40 |
| 1. Concepts | 40 |
| 2. Methods and Approaches | 42 |
| 3. Summary | 57 |
| Transcriptional Regulation of Gene Expression | 58 |
| 1. Concepts | 58 |
| 2. Methods and Approaches | 69 |
| 3. Summary | 107 |
| Posttranscriptional Processing of Messenger RNA | 114 |
| 1. Concepts | 114 |
| 2. Methods and Approaches | 125 |
| 3. Summary | 145 |
| Study Questions | 150 |
| References | 152 |
| Part II REGULATION OF PROTEIN LEVELS AND TRANSCRIPTION FACTOR FUNCTION | 156 |
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| Overview | 158 |
| Protein Synthesis and Turnover | 164 |
| 1. Concepts | 164 |
| 2. Methods and Approaches | 166 |
| 3. Summary | 168 |
| Proteomics | 170 |
| 1. Concepts | 170 |
| 2. Methods and Approaches | 171 |
| 3. Summary | 181 |
| Determination of Protein–Protein Interactions and the Motifs That Mediate Them | 182 |
| 1. Concepts | 182 |
| 2. Methods and Approaches | 182 |
| 3. Summary | 223 |
| Posttranslational Modifications | 224 |
| 1. Concepts | 224 |
| 2. Methods and Approaches | 229 |
| 3. Summary | 246 |
| Dissection of Signaling Pathways | 248 |
| 1. Concepts | 248 |
| 2. Methods and Approaches | 249 |
| 3. Summary | 266 |
| Study Questions | 268 |
| References | 270 |
| Part III USE OF TRANSGENIC AND KNOCKOUT MICE TO STUDY GENE REGULATION | 274 |
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| OVERVIEW | 276 |
| Production of Transgenic Animals | 280 |
| 1. Standard Production | 280 |
| 2. Applications of Transgenic Models | 287 |
| KNOCKOUT ANIMALS | 304 |
| 1. Standard Production | 304 |
| 2. Applications of Null Mouse Models | 309 |
| Isolation of Cells and Cell Lines From Transgenic and Knockout Animals | 328 |
| 1. Standard Production | 328 |
| Study Questions | 330 |
| References | 332 |
| Index | 338 |