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Regulation of Gene Expression

:	Gary H. Perdew, Jack P. Vanden Heuvel, Jeffrey M. Peters
:	Regulation of Gene Expression
:	Humana Press
:	9781597452281
:	1
:	CHF 85,30
:

:	Biochemie, Biophysik
:	English

:	334
:	Wasserzeichen/DRM
:	PC/MAC/eReader/Tablet
:	PDF

The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select 'Courses. ' The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

	Preface	5
	Contents	7
	COMPANION CD	9
	Part I GENE EXPRESSION CONTROL AT THE MRNA LEVEL	10
	Messenger RNA Accumulation	20
	1. Concepts	20
	2. Basic Methods and Approaches	23
	3. Summary	38
	Transcript Profiling	40
	1. Concepts	40
	2. Methods and Approaches	42
	3. Summary	57
	Transcriptional Regulation of Gene Expression	58
	1. Concepts	58
	2. Methods and Approaches	69
	3. Summary	107
	Posttranscriptional Processing of Messenger RNA	114
	1. Concepts	114
	2. Methods and Approaches	125
	3. Summary	145
	Study Questions	150
	References	152
	Part II REGULATION OF PROTEIN LEVELS AND TRANSCRIPTION FACTOR FUNCTION	156
	Overview	158
	Protein Synthesis and Turnover	164
	1. Concepts	164
	2. Methods and Approaches	166
	3. Summary	168
	Proteomics	170
	1. Concepts	170
	2. Methods and Approaches	171
	3. Summary	181
	Determination of Protein–Protein Interactions and the Motifs That Mediate Them	182
	1. Concepts	182
	2. Methods and Approaches	182
	3. Summary	223
	Posttranslational Modifications	224
	1. Concepts	224
	2. Methods and Approaches	229
	3. Summary	246
	Dissection of Signaling Pathways	248
	1. Concepts	248
	2. Methods and Approaches	249
	3. Summary	266
	Study Questions	268
	References	270
	Part III USE OF TRANSGENIC AND KNOCKOUT MICE TO STUDY GENE REGULATION	274
	OVERVIEW	276
	Production of Transgenic Animals	280
	1. Standard Production	280
	2. Applications of Transgenic Models	287
	KNOCKOUT ANIMALS	304
	1. Standard Production	304
	2. Applications of Null Mouse Models	309
	Isolation of Cells and Cell Lines From Transgenic and Knockout Animals	328
	1. Standard Production	328
	Study Questions	330
	References	332
	Index	338